As part of my research activities, currently I am involved in HPLC method development for redox- active analytes and GC-MS analysis of F2-isoprostanes. Apart from my scientific research, I mentor lab staff, train lab technicians and assistants. I ensure the overall laboratory operate smoothly.
Shylesh Bhaskaran, Ph.D.
Associate Staff Scientist
I received my Ph. D. in Biochemistry from the Mahatma Gandhi University, India. I completed my postdoctoral training at the Louisiana State University Health Science Center, New Orleans and University of Texas Health Sciences Center at San Antonio, TX. My research focuses on characterizing the role of neuronal oxidative stress in age-associated muscle loss (sarcopenia) in mouse models.
Jacob Brown, Ph.D.
My career has focused on delineating novel mechanisms of skeletal muscle wasting and changes in skeletal muscle metabolism in (patho)physiological conditions such as aging, disuse, cancer and obesity. I earned my Ph.D. at the University of Arkansas where I was mentored by Dr. Nicholas P. Greene. Currently, I am pursuing post-doctoral research training under the guidance of Dr. Holly Van Remmen at Oklahoma Medical Research Foundation, where I am exploring the role of oxidative stress and lipid hydroperoxides in sarcopenia.
Bumsoo Ahn, Ph.D.
I am a redox biologist with an interest in mitochondria and muscle. I try to understand the mechanisms underlying sarcopenia (age-related loss of muscle mass and function) using genetic knockouts and transgenic mice. I am also working to discover a pharmacological intervention that can delay sarcopenia and thus extend our healthy lifespan (i.e. healthspan).
Gavin Pharaoh is a senior graduate student focusing on the role of mitochondria in aging. His dissertation explores the role of hydrogen peroxide and lipid hydroperoxides in denervation muscle atrophy. He has also worked on projects including how novel breast cancer treatments affect mitochondrial function, how Nrf2 transcription factor affects muscle aging and mitochondrial function, how mitochondrial chaperones influence lifespan and disease, and how insulin-like growth factor 1(IGF-1) signaling affects mitochondria in the aging brain and periphery.
I’m a PhD Candidate in Neuroscience at OUHSC. My background is in Biotechnology (MSc from Jagiellonian University, Poland), and I gained international lab experience during internships, such as a summer internship at Karolinska Institutet (Sweden) and participating in Visiting Research Graduate Traineeship Program at Oklahoma Medical Research Foundation (USA). I study spinal cord aging, especially the changes that occur in aging alpha-motor neurons and how to prevent their age-related decline and eventual loss. A big part of my project is confocal microscopy and image analysis, which I enjoy very much.
Senior Research Technician
I manage our very large animal colony. This involves weaning, collecting DNA/tissues, setting up breeders, euthanasia, health checks, checking for new litters and injections.
As a research technician, I assist the post-docs and staff scientists on their various research projects. My specialties include Western blotting, animal sacrifice & dissection, mitochondria isolation and immunofluorescence staining. I was recently accepted to the OU College of Medicine Class of 2023, which I will start attending in August 2019.
I primarily use GC-MS to analyze levels of F2-Isoprostanes in several tissue types. We believe this species is a strong indicator of cellular oxidative stress. I am also involved in tissue harvesting, western blotting, RT-PCR and genotyping for the lab.
As a laboratory technician, I assist other scientists in our lab with a variety of projects. I am involved with genotyping, activity assays, Western blotting, and animal handling. I am excited to learn more as we explore different factors that contribute to muscle loss in aging.
Assist on trainees’ research projects by conducting specialized assays on mice such as GTT/ITT, QMR, genotyping and tissue collection. Also, perform different mitochondrial functional/stress assays using O2K and Seahorse on mice tissues, and primary as well as established cell lines.
As a research technician in the Aging & Metabolism department, I help study the question of why we lose muscle as we get older. My role in answering this question is to perform various types of lab work to collect data about this topic. I assist in keeping records, identifying & preparing samples, and performing tasks such as mouse handling, PCR genotyping, denervation surgeries, drug injections and mitochondrial isolation. This assists the research staff in understanding the neurobiology and mitochondrial function in mice at different stages of their life.